Al Qaeda-linked insurgent group al Shabaab is seeking to topple the government and establish its own rule in Somalia based on its own strict interpretation of Islamic sharia law.
In a statement, the Pentagon said the strike took place near Galkayo, Somalia.
While the United States has frequently carried out air strikes in Somalia against al Shaabab, this was the first since Jan. 20 when Biden took office.
The group's campaign of bombings and gun attacks has targeted Somali military bases and civilian infrastructure including hotels, bars and schools in both Somalia and other regional countries.