Harry and Meghan, known officially as the Duke and Duchess of Sussex, host the episode that features reflections and hopes for the new year from singer Elton John, producer and actor Tyler Perry, tennis champion Naomi Osaka and other celebrities.
In excerpts released by Spotify, the couple thanked healthcare and frontline workers and others who made sacrifices during a challenging year.
"From us, I'll say no matter what life throws at you guys, trust us when we say, love wins," Meghan said.
"Love always wins," Harry added.
Harry and Meghan stepped back from their royal duties in January and moved to Southern California with their infant son Archie. In September, they signed a multi-year production deal for TV programming with Netflix Inc, followed by a multi-year agreement to produce and host podcasts for Spotify.