While physicists continue to argue about whether time is indeed an illusion as Albert Einstein suggested, biologists have no doubt about its significance for understanding life as a dynamic system.
In recent years, they have been gaining an increasingly deeper understanding of complex biological systems using tools making possible the simultaneous analysis of vast amounts of cellular and molecular data and the probing of cellular circuitry that drives disease.
But these in-depth investigations of how cells behave and interact have provided only separate snapshots of what happens inside complex organisms, without accounting for the dimension of time and revealing the sequence of cellular events.
Now, researchers at the Weizmann Institute of Science in Rehovot have developed the first technique to measure cellular changes in the body over time. The method, called Zman-seq (zman means “time” in Hebrew) reveals the history of cells and may advance the development of new therapies for cancer and other disorders.
Now, in a new study they just published in Cell under the title “Time-resolved single-cell transcriptomics defines immune trajectories in glioblastoma,” researchers from Prof. Ido Amit’s immunology department lab have managed for the first time to develop a method for tracking and measuring changes over time on in single cells inside the body.
The method consists of labeling cells with different time stamps and tracking them in healthy or pathological tissue. Using this “cellular time machine,” researchers can get to know the cells’ history and how long each cell had stayed in the tissue, ultimately achieving an understanding of the molecular and cellular temporal changes that had taken place within that tissue.
Single-cell technology innovation
Single-cell technologies, the tools that enable biologists to understand what happens inside individual cells have advanced significantly in recent years, in large part thanks to the vibrant single-cell research community in which Amit’s lab is one of the pioneers.
With these tools, it is now possible to obtain high-resolution images of how diseases develop and how the body responds to different medications, to identify rare cell populations, identify which cells interact with one other, and how they are spatially distributed in a tissue.
However, all these important insights are equivalent to getting many still-frame images from a movie and trying to understand the plot.
“Knowing what preceded what is not enough to deduce causality, but without this knowledge, we don’t really have a chance of understanding what the cause is and what is the effect,” Amit said.
The development of the groundbreaking new technology started with the research of Dr. Daniel Kirschenbaum, a postdoctoral researcher in Amit’s lab. Born in Hungary, he completed his doctoral degree in neuropathology in Switzerland, where he studied glioblastoma, the most common and aggressive brain tumor.
“We usually think of cancer as cells growing out of control, but in fact, cancer is also the loss of the ability of the body, and specifically of its immune system, to control this growth,” Kirschenbaum explained. “And when you look at tumors, large parts of them are composed of dysfunctional immune cells, which sometimes make up one-third or even half of all the cells in a tumor.”
Defeating Glioblastoma
Glioblastoma is one of the most immune-suppressive types of tumors.
“To understand how to defeat this cancer, we need to understand what happens to the immune cells as they enter the tumor and why they lose the capacity to fight the tumor and become dysfunctional,” he added.
“Ideally, we’d want to have a little clock on each cell telling us when it entered the tumor and when the signals and checkpoints that instruct it to become incompetent are activated. This back-to-the-future time machine was thought to be impossible to develop.”
The breakthrough came when Kirschenbaum decided to take an uncanny approach.
“Instead of trying to measure time in cells within the tumor tissue, we decided to try to mark the cells while they are still in the blood – before they enter the tumor. By using different fluorescent dyes at different time points, we are later able to know exactly when each cell entered the tissue and how long it had been there, and this reveals the dynamic changes that happened to the cells in the tissue, for example, what are the different stages at which immune cells become dysfunctional inside the tumor.”
The challenge, he said, was to develop the optimal way to color the cells in the blood at specific time points, making sure the dye does not reach the tissue itself or stay too long in the blood, potentially mixing with the next dye. At the same time, the dye had to stay on the cells long enough for them to be measured.
As part of the study, the researchers in Amit’s lab showed that the method makes it possible to measure time in immune cells in different tissues – the brain, the lungs, and the digestive system of animal models.
Using Zman-seq, the team was able to gain insights into why the immune system is so dysfunctional in battling glioblastoma. “For example, we showed that immune cells called natural killer cells, which, as their name implies, are crucial to killing rogue cells, become dysfunctional very quickly because the tumor hijacks their killing mechanisms – and this happens within less than 24 hours after they enter into the tumor.
This explains why therapeutic attempts to harness the immune system for fighting glioblastoma are so ineffective,” Kirschenbaum says.
Now, they are developing ways to block the immune-disabling tumor checkpoints to reactivate the immune system in glioblastoma and other hard-to-treat tumors. In addition, they plan to adapt Zman-seq to the study of temporal dynamics of cells throughout the human body.
“For example, many cancer patients are getting therapy before surgery. We want to use the method to color immune cells in the body during that period so that after the surgery, we can better understand the dynamics of immune cells in the tumor and optimize patient treatments,” Kirschenbaum noted.
“Until today, there were quite a few different methods trying to analyze single-cell data and arranging them along a time axis according to different parameters. But these approaches were all somewhat arbitrary in choosing what is the sequence of events,” Amit concluded. “Zman-seq supplies the ‘hard facts,’ the empirical measurements enabling scientists to understand the precise order of events that immune and other cells are going through when they enter a tumor, and this may lead to completely new thinking on how to generate more effective therapies for cancer and other disorders.”