The Israeli embassy in Spain said in a statement that Israel strongly condemned recent remarks by some members of the Spanish government, whom it did not specify.
She was defeated by Xavier Trias, the right-wing candidate from the Junts party, a former mayor who Colau had previously unseated in 2015.
The fire was started when a waiter flambéed a pizza and set fire to the walls and ceiling.
FC Barcelona's president Joan Laporta invited representatives of the Israeli embassy in Spain to their soccer match against Manchester United.
Isabel Díaz Ayuso, head of the Community of Madrid, decided to visit Israel in response to Barcelona ending its ties with Tel Aviv.
The ambassador was evacuated by security guards while dozens of activists were swarming her - in spite of this, she finished her lecture.
Twin or sister cities typically collaborate on tourism enterprises. Barcelona launched two initiatives last year to draw Jewish and Israeli tourists.